Begin with pickup at your centrally-located Panama City hotel, then make the 1.5-hour trip to to Colón in an air-conditioned minivan or minibus. See the rainforest from the water on a 20-minute ferry ride, then go deeper into the forest by vehicle, with plenty of stops to sight wildlife: watch for sloths, monkeys, anteaters, and other mammals in the thick trees.
Continue to San Lorenzo Fort (entrance fees not included), which guarded the Caribbean end of the Las Cruces Trail, the overland route for Spanish treasures and gold from Central and South America. Learn the story of how the fort finally fell to the pirate Henry Morgan, who used it as a base for his historic attack on Panama City.
After visiting the fort, tour the Agua Clara section of the Panama Canal extension (entrance fees not included), which was inaugurated in summer, 2016. Find out how engineers raised the waterline of Gatun Lake, built new locks, and widened existing channels to accommodate New Panamax ships. You’ll have roughly 45-minutes to explore the locks before returning to Panama City, where this tour concludes with drop-off at your hotel.