Begin your day with pickup from St Paul's Cathedral on Flinders St, then travel by comfortable air-conditioned van for approximately one hour to reach the Yarra Valley.
Spend approximately 2.5 hours at Healesville Sanctuary, home to native Australian animals such as kangaroos, koalas, wombats, and emus. See the animals in spacious enclosures that replicate their natural habitat, and learn about the care of injured wildlife at the on-site Australian Wildlife Heath Centre, where veterinarians treat sick and orphaned critters.
Once you have seen the animals, head to a local winery for lunch at their restaurant and a wine tasting that lasts approximately 1.5 hours. Save some appetite for a treat at the Yarra Valley Chocolaterie and Ice Creamery. Try some of their huge array of chocolates and perhaps purchase something to take home.
Your tour ends with drop-off at your starting point in Melbourne in the late afternoon.